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KMID : 0369820070370010039
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Jorunal of Korean Pharmaceutical Sciences
2007 Volume.37 No. 1 p.39 ~ p.44
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Encapsulation of Plasmid DNA in PLGA Nanoparticles : Effects of Poloxamer and Temperature
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Park Jeong-Sook
Kang Hyun-Sook
Ryu Sang-Hwa
Myung Chang-Seon
Hwang Sung-Joo
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Abstract
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Previously, we have reported that PLGA nanoparticles were prepared for sustained released of water-soluble blue dextran and the particle size, in vitro release pattern and encapsulation were modulated by varying polymers. This study was designed to encapsulate plasmid DNA in PLGA nanoparticles and to investigate the effect of polymers and tem- peratures. PLGA nanoparticles were fabricated with poloxamer 188 (P188) or poloxamer 407 (P407) by using spontancous emulsification solvent diffusion method. As a model plasmid DNA, pCMV-Taq2B/IL-18 was encapsulated in PLGA nano- particles. Then, the particle size, zeta potential and encapsulation efficiency of nanoparticles containing plasmid DNA were investigated. Particle sizes of PLGA nanoparticles prepared with P188 and P407 were in the range of 200-330 nm and 250- 290 nm, respectively. Zeta potentials of nanoparticles were negative regardless of nanoparticle compositions. Encapsulation effeiency of P407 nanoparticles prepared at 30℃ was higher than those at other preparation condition. From the results, the PLGA nanoparticles prepared with poloxamers at different temperature, could modulate the particles size of nano- particles, and encapsulation efficiency of plasmid DNA.
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KEYWORD
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PLGA nanoparticles, Poloxamer DNA, Encapsulation
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